FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065657
Sequences flagged as poor quality0
Sequence length8-150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1419494.630296213829531No Hit
NNNNNNNN510121.6639826308031198No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT305000.994892775023429No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT196460.6408414248560749No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC185510.6051231432609714No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA173670.5665017319289144No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG110880.36168429801507473No Hit
CGGCCCCCCACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC107680.35124607873614044No Hit
ATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTC102420.3340882557963921No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC90550.295368986158595No Hit
CGGCCCCCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA83490.27233966487444616No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG81650.2663376887890589No Hit
CGGCCCCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATG81330.26529386686116546No Hit
CGGCCCCCCATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG68210.2224971678175347No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG64960.21189585136236702No Hit
TATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAA64540.21052583508200687No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA60740.19813044968827234No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG55230.18015714086735732No Hit
CGGCCCCCCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC52970.17278514850160995No Hit
AGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACT52520.17131727391550977No Hit
ATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAAT52410.17095846012779642No Hit
CGGCCCCCCAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGAC51810.16900129401299624No Hit
CGGCCCCCCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGC51740.16877295796626954No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA50800.1657067310530826No Hit
CGGCCCCCCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGA50150.16358646776204905No Hit
CGGCCCCCCATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCT47450.1547792202454482No Hit
GGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGA47440.15474660081020153No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG47260.15415945097576148No Hit
CGGCCCCCCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAT46680.15226752373145463No Hit
CGGCCCCCCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCC45910.14975582721746106No Hit
CGGCCCCCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGC44870.14636340595180738No Hit
CATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGT43600.14222073767548032No Hit
CCCCCGTCTAGAGGCTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT42490.13859998036309998No Hit
CGGCCCCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGA41480.13530541740318633No Hit
CGGCCCCCCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAT40140.13093441308013257No Hit
CGGCCCCCCGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTAC40130.1309017936448859No Hit
CAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTAC37960.12382337619635855No Hit
CGGCCCCCCGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTG37790.12326884579716517No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG36530.11915879695608479No Hit
TGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTG36220.11814759446343802No Hit
GCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGC35410.11550542020845776No Hit
CGGCCCCCCGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAA34110.11126489362639068No Hit
CGGCCCCCCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTG34090.11119965475589735No Hit
CGGCCCCCCACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGG33500.10927510807634383No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGG32500.10601316455167685No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC31850.10389290126064331No Hit
CGGCCCCCCAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACT31770.10363194577866995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)