FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065657
Sequences flagged as poor quality0
Sequence length8-150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1442224.704440190145212No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT171660.5599452254443338No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT130810.42669483246168766No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG114240.3726444282579558No Hit
CGGCCCCCCACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC80230.26170572898403177No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC80180.26154263180779846No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA72260.23570803909243598No Hit
TATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAA70090.22862962164390865No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC64140.20922105767214008No Hit
CGGCCCCCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA62140.20269717062280615No Hit
CGGCCCCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATG61600.20093572111948596No Hit
ATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTC52100.16994725763514965No Hit
CGGCCCCCCATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG51280.16727246394492273No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA46670.15223490429620795No Hit
CGGCCCCCCCCGTCTAGATCCTCGGTACTACATGCTTAGTCAGTCTTTAC45180.14737460844445416No Hit
CCCCCGTCTAGAGGCTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT44060.14372123169682716No Hit
CGGCCCCCCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC42310.13801283052865992No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG41560.13556637288515969No Hit
CGGCCCCCCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGA39730.12959701623501912No Hit
GCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGC39690.12946653849403245No Hit
CAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTAC37620.12271431539797178No Hit
CGGCCCCCCCCCGTCTAGATCCTCGGTACTACATGCTTAGTCAGTCTTTA36550.11922403582657812No Hit
CGGCCCCCCATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCT36240.11821283333393136No Hit
CGGCCCCCCAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGAC35200.1148204120682777No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG34860.11371135126989093No Hit
CGGCCCCCCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTG34070.11113441588540401No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG31850.10389290126064331No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC30790.10043524112449632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)