FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4796929
Sequences flagged as poor quality0
Sequence length8-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1852763.8623877901882637No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC266100.5547299115746762No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG235400.4907306320356211No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT190490.3971082332050359No Hit
TATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAA146690.3057998148398694No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT127890.26660807362377054No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA124280.2590824254434452No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA116200.2422383153888665No Hit
CGGCCCCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATG98060.20442245444950302No Hit
CGGCCCCCCACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC90990.18968385815174665No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC83120.17327752818522016No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC79530.16579357334661407No Hit
CGGCCCCCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA75870.15816369181199053No Hit
CGGCCCCCCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC72800.15176376385808504No Hit
CGGCCCCCCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTG69780.14546806925847766No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG66590.13881798125425662No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG62500.13029169287266915No Hit
CGGCCCCCCATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG61300.1277900923695139No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG59020.12303705141351895No Hit
GTATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTA58540.12203641121225685No Hit
AGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACT57270.1193888840130842No Hit
CGGCCCCCCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGA56830.1184716304952606No Hit
GCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGC56130.1170123635350867No Hit
CGGCCCCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGA54940.11453160970279111No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTA54550.11371858953926564No Hit
CGGCCCCCCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGC54160.11290556937574019No Hit
CGGCCCCCCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCC52640.10973687540507687No Hit
CCCCCGTCTAGAGGCTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT50600.10548415454971297No Hit
CGGCCCCCCCCGTCTAGATCCTCGGTACTACATGCTTAGTCAGTCTTTAC49330.10283662735054032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)