FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4136983
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT2186695.285711833962092No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT232110.5610610437606343No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT199310.48177621227836803No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA167080.40386919646515346No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC148190.35820790174868977No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG143910.3478621981284429No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA101160.2445260229495746No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC88080.21290877917554893No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG81830.197801151225422No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA67160.16234052690088405No Hit
CGGCCCCCCACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC63740.15407363288657458No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC60380.14595177210058635No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG57940.1400537541488568No Hit
CGGCCCCCCATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCT56610.1368388509210698No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT55400.13391401414992518No Hit
CGGCCCCCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA50860.122939833206953No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG48980.11839545871955481No Hit
TATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAA48320.1168000932080214No Hit
CGGCCCCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATG47440.11467293919264353No Hit
CGGCCCCCCATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG44870.10846068257955133No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT44330.10715538352466036No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA41840.10113650454932979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)