FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605282
Sequences flagged as poor quality0
Sequence length8-150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1639744.548160171659249No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT165560.4592151182625936No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT163880.45455528860155736No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC163030.4521976366897236No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG151940.42143721351062136No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA142450.3951147233420298No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG121310.33647853344065737No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC115670.32083481957860716No Hit
TATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAA91450.2536556086320016No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA81250.22536378568999596No Hit
CGGCCCCCCACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC76930.2133813665616171No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG70100.19443694002299958No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG69990.19413183212852697No Hit
CGGCCCCCCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC64850.17987497233226138No Hit
CGGCCCCCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA64500.17890417448621218No Hit
ATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTC61560.17074947257939876No Hit
CGGCCCCCCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGC57140.158489682637863No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG56180.15582692283155658No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAAC52690.14614668145238016No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG48020.13319346447795208No Hit
CGGCCCCCCATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG47880.1328051453395324No Hit
CGGCCCCCCCCGTCTAGATCCTCGGTACTACATGCTTAGTCAGTCTTTAC46520.1290329022805983No Hit
CGGCCCCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATG45810.1270635695071842No Hit
GCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGC44650.12384606807456394No Hit
CGGCCCCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGA43460.1205453553979966No Hit
CGGCCCCCCGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTAC43400.12037893291010245No Hit
CGGCCCCCCAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGAC42830.11879791927510804No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT41840.11605194822485454No Hit
CGGCCCCCCAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACT41200.11427677502065027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)