FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7395382
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1654402.237071729357591No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA1433991.9390343865942288No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG761411.02957494284947No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT675080.912839931730369No Hit
GCCAGGATCAAACTCTCAAGTTGGACTTGAAACCTTTGAACCGGCTGATC611510.8268808832322658No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA463850.6272157408501684No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG431700.5837426653552177No Hit
TGGCGGAGCTGGCCGAGGAATACGAGGACGTGCGCCGACGCCACGCCAAC430570.5822146847857217No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT396410.5360236969503401No Hit
CGGCCCCCCACTGAGCGCGGCGCCGCCGGGTCACTGACCAAGGCGAGCGA375010.5070867197935144No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT347050.46927934216244677No Hit
AGAAATCTGGAACCTCAAGCGCGGCGGCCACGATTACCGCAAGGTCTACG313760.4242647641460576No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC266860.360846809536005No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG256430.34674341366003814No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT247760.33501988132594096No Hit
CGGCCCCCCACGGGGGCCTGCACAAGCGGTGGAGCATGTGGTTTAATTCG235140.3179551779745793No Hit
AGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGTGATCCGCA219070.2962254012030751No Hit
CGGCCCCCCTCAGCACCTCGCTGCGGCTCTCGTCCTTGTAGACCTCGATG216500.2927502595538675No Hit
AGGCCTTGACCGCGTACTTCCGCAGCGGGTACAGCCGCTCCTTCCGCTGC193460.26159568227848135No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG187550.25360420868049816No Hit
CGGCCCCCCAACGGGCACCTCATCAACGGGCAGGAAGTACCCGATATTGC163770.22144900696137132No Hit
CGGCCCCCCAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGAC163270.2207729093642492No Hit
AGGCCCCGCCGACCCTTCCCCCACGGTCCCTCTCTCTTCACCAGCCCCTC144400.1952569860488613No Hit
CGGCCCCCCACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACG143910.19459441040368164No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG141450.19126801022584095No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT137980.1865758929018136No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG137290.1856428782177851No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG136290.1842906830235409No Hit
GCCTTCAGCAATCAATTCCATTCATACATCAGCACTCTGCTGGTTCTGTT132650.17936869251649204No Hit
CGGCCCCCCACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGG131920.1783815900246938No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACTGAGCGCGGCGCCGCCGGGTC129660.1753256288857019No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT128340.17354073122929958No Hit
AACAGGTGTGAAGACTCAAGATGCGCACACAGACGCTGTCCGTGGTTTTA124140.16786151141347397No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG120800.16334517946469837No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG118060.1596401646324693No Hit
CGGCCCCCCGCCTTGTGCCGCCGTTCCGGGACGAAGCCGCCGAGGGCCTT117990.1595455109688722No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA114640.15501565706815415No Hit
CGGCCCCCCGACTTCCTCTTTCCCTCGGAGCGGGCGGCGGCGTTGGCGGC111630.15094554953347913No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA111080.15020184217664484No Hit
GTGTCCCGCCCTACTCAAGGACTTTTGCTATTCTACGTGTACGGGGCTGT110060.14882260307851575No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG108010.14605060293031516No Hit
TCGGCGTCACCGAGCTCAAGCCGAAGAATGGCTGGTACGATTATGACGCC104610.1414531392698849No Hit
AGGCTGGGAACTGAAGAGACTTGTGGTATTCCATCTCGGGTGCCTCTGTT104500.14130439779851806No Hit
GAAATCCTGTCTGAACATGGGTCGACCACGATCCAAGCCTAAGTACTCGT102750.1389380562085907No Hit
ATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTC102660.13881635864110875No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC97890.13236638756456395No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT95170.12868841663621974No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG94870.12828275807794648No Hit
CGGCCCCCCTTCGGGGGCATTACCCATGGGCCCCCTGAGAAGAAGATGGG93950.12703873849924183No Hit
CCAGGATCAAACTCTCAAGTTGGACTTGAAACCTTTGAACCGGCTGATCA93610.1265789921331988No Hit
CGGCCCCCCAAAATGGAGGGGATGAGCTGTGGATAGGGGTGAAAGGCCAA92420.12496987985204822No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG90720.1226711480218331No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG90150.1219003967611139No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTCAGCACCTCGCTGCGGCTCTCG89690.12127838697176156No Hit
CGGCCCCCCGGTTTTTGTTGCTGCGCCGCCACCGCCCGAGTCATGTTCCG88520.11969631859449585No Hit
CAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATA88140.11918248442068308No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT82570.11165075718874293No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG78730.10645832764284521No Hit
GACGGTTATTTGCCGCCCGCTGAAATCCAGGAAGCTGCGCCGACCGCGGG77450.10472751779421266No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG75500.10209073716543647No Hit
GGAATTCCTGGTGGAGCGGTGGAATGCGCAGATATCAGGAGGAACACCAG74740.1010630688178109No Hit
CGGCCCCCCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGA74500.1007385419711923No Hit
CGGCCCCCCTGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGA74180.10030583950903416No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT74030.10010301022989752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)