FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7395382
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1772892.3972933379235855No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA926701.2530792865060927No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG572660.7743480999358789No Hit
GCCAGGATCAAACTCTCAAGTTGGACTTGAAACCTTTGAACCGGCTGATC528770.7150002528605013No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT443210.5993064320409682No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT420970.5692336109209775No Hit
CGGCCCCCCACTGAGCGCGGCGCCGCCGGGTCACTGACCAAGGCGAGCGA406600.5498025659796884No Hit
TGGCGGAGCTGGCCGAGGAATACGAGGACGTGCGCCGACGCCACGCCAAC382030.5165791300571085No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT343300.46420861018403103No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG283490.38333381561628593No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA246930.3338975593147183No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG240840.32566269058177116No Hit
CGGCCCCCCTCAGCACCTCGCTGCGGCTCTCGTCCTTGTAGACCTCGATG219190.2963876646263844No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC218450.2953870401826437No Hit
AGAAATCTGGAACCTCAAGCGCGGCGGCCACGATTACCGCAAGGTCTACG216870.2932505717757379No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT203640.27536102935588724No Hit
AGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGTGATCCGCA180890.24459858868683187No Hit
AGGCCTTGACCGCGTACTTCCGCAGCGGGTACAGCCGCTCCTTCCGCTGC171430.2318068221492818No Hit
CGGCCCCCCAACGGGCACCTCATCAACGGGCAGGAAGTACCCGATATTGC157160.2125109967274172No Hit
AGGCCCCGCCGACCCTTCCCCCACGGTCCCTCTCTCTTCACCAGCCCCTC155910.21082075273461195No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG148390.20065224487389563No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT145040.19612239097317757No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG137640.18611614653577055No Hit
CGGCCCCCCACGGGGGCCTGCACAAGCGGTGGAGCATGTGGTTTAATTCG136980.18522369770756938No Hit
CGGCCCCCCAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGAC130460.17640738504109726No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG128160.17329733609433562No Hit
CGGCCCCCCGCCTTGTGCCGCCGTTCCGGGACGAAGCCGCCGAGGGCCTT126290.170768731081099No Hit
CGGCCCCCCACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACG121850.16476498441865478No Hit
CGGCCCCCCGACTTCCTCTTTCCCTCGGAGCGGGCGGCGGCGTTGGCGGC119550.16165493547189314No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT117200.15847727676541928No Hit
GTGTCCCGCCCTACTCAAGGACTTTTGCTATTCTACGTGTACGGGGCTGT113020.15282510085347858No Hit
CGGCCCCCCACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGG109960.14868738355909134No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG106830.14445501260110702No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACTGAGCGCGGCGCCGCCGGGTC103580.1400603782198134No Hit
CACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTTG100640.13608492434873548No Hit
AGGCTGGGAACTGAAGAGACTTGTGGTATTCCATCTCGGGTGCCTCTGTT100430.13580096335794417No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG99770.134908514529743No Hit
AACAGGTGTGAAGACTCAAGATGCGCACACAGACGCTGTCCGTGGTTTTA96030.12985130450326976No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC94960.12840445564542846No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT91310.12346894318643716No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC84810.1146796744238499No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG84130.11376018169176386No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG82360.11136679619795165No Hit
CCAGGATCAAACTCTCAAGTTGGACTTGAAACCTTTGAACCGGCTGATCA82180.11112340106298768No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG80580.10895988875219698No Hit
GAAATCCTGTCTGAACATGGGTCGACCACGATCCAAGCCTAAGTACTCGT79050.10689103010500337No Hit
CGGCCCCCCGGTTTTTGTTGCTGCGCCGCCACCGCCCGAGTCATGTTCCG78750.1064853715467301No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT77430.10470047389032777No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTCAGCACCTCGCTGCGGCTCTCG76470.10340236650385336No Hit
GCCTTCAGCAATCAATTCCATTCATACATCAGCACTCTGCTGGTTCTGTT76250.10310488356111963No Hit
CGGCCCCCCCACTGAGCGCGGCGCCGCCGGGTCACTGACCAAGGCGAGCG76060.10284796647421324No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG75460.10203664935766671No Hit
CGGCCCCCCTTCGGGGGCATTACCCATGGGCCCCCTGAGAAGAAGATGGG74980.10138759566442951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)