FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510643
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA548031.214970903261464No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA329980.7315586713468568No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC294370.6526120555317723No Hit
NNNNNNNN222050.4922801471985258No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC122620.27184594302852166No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC114210.253201151144083No Hit
TCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTA88780.1968233797265711No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG84090.1864257490561767No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT82920.1838318838356305No Hit
TGGCTGGACGATGCCTCGGCTTGACCTTTTTTGAAAAGTGCTGGTTAGTT79520.1762941558442998No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT78120.17319038549492835No Hit
CTCAAACTCCTGATCTCAAGTGATCCACCGGCCTCGGCCTCCCAAAGTGT75340.16702718437260497No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC71730.15902389082886853No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT70460.1562083277262244No Hit
TGGCTGGGATGGCAGCACCCTTCTTACCAGGAGTTGCTACCAATGCTTTG69810.15476729149258764No Hit
CCTGGGATCCTGCACTCAAGCCATAATTCTGCCACTTAGCCTGACTGCCG69150.1533040854707411No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT67190.14895880698162103No Hit
AGGCGAAGACTGGCTAATTGACTTAGCAAGTAGCCATGCTGAAATTCAGC65560.14534513150342424No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG59140.13111212747273504No Hit
TGGCATTCGCCAACGAGCCAGAGCCACTTTTCGGATCCGTCACTTCCAGC58710.1301588265797138No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG57900.1283630737347203No Hit
AGGCATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTG57580.12765364051200684No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT55070.12208902367134797No Hit
AGGCGCGGGGTGCTAAGGTCACGCGCCGAGAGGGAAAGAACCCAGACCAT54820.12153477896610307No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA54650.12115789256653652No Hit
TGGCGCCACTACTTCCAGAACACACAAGGCCTGATCTTCGTGGTGGACAG53720.11909610226302549No Hit
CTGGGATCCTGCACTCAAGCCATAATTCTGCCACTTAGCCTGACTGCCGG51520.11421874885687029No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG50950.11295507092891192No Hit
CCTCCCCTACCGCACTCAAGCCGGCCCGTACCCACCGCAAGCCCCCAGTT50270.11144752533064575No Hit
GGAACTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAAGAACACCGG49420.1095630933328131No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT49000.10863196222800164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)