FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510643
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA333330.7389855503971385No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC255590.5666376168541825No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA194720.4316901160211526No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC130450.28920488719679216No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC100580.2229837298141307No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT82150.18212481014347623No Hit
TGGCTGGACGATGCCTCGGCTTGACCTTTTTTGAAAAGTGCTGGTTAGTT75060.1664064303027307No Hit
TCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTA73640.1632583203769396No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT64880.1438375859051581No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG61670.1367210838898135No Hit
TGGCATTCGCCAACGAGCCAGAGCCACTTTTCGGATCCGTCACTTCCAGC58850.13046920361465095No Hit
TGGCTGGGATGGCAGCACCCTTCTTACCAGGAGTTGCTACCAATGCTTTG58660.13004797763866482No Hit
CCTCCCCTACCGCACTCAAGCCGGCCCGTACCCACCGCAAGCCCCCAGTT57800.12814137585262236No Hit
CTCAAACTCCTGATCTCAAGTGATCCACCGGCCTCGGCCTCCCAAAGTGT57620.127742319664846No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG57500.12747628220632845No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA56580.1254366616910272No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT56070.1243060024923276No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT55400.12282062668227126No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT54840.12157911854252265No Hit
AGGCATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTG53690.11902959289839608No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC53270.11809846179358463No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG52580.1165687464071087No Hit
AGGCGAAGACTGGCTAATTGACTTAGCAAGTAGCCATGCTGAAATTCAGC50960.11297724071712172No Hit
CCTGGGATCCTGCACTCAAGCCATAATTCTGCCACTTAGCCTGACTGCCG49460.10965177248565226No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG45850.10164847894191584No Hit
TGGCGCCACTACTTCCAGAACACACAAGGCCTGATCTTCGTGGTGGACAG45470.10080602698994355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)