FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4759912
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC488451.0261744334769214No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA414230.8702471810403218No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC119160.25034076260233384No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA99670.209394627463701No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG90020.18912114341609676No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG82950.17426792764235977No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG76850.1614525646692628No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT72490.15229273146226233No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC69420.14584303239219548No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC59830.1256956010951463No Hit
CGGCCCCCCGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCT58050.12195603616201307No Hit
GGGCACCGGTGAATCCAAGTGTCCTCTGATGGTCAAAGTTCTAGATGCTG54050.11355251945834294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)