FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892940
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTT339430.8719117171084065No Hit
GTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACGTTCGGAAACGGA297060.7630736666889292No Hit
AGAACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGATGCTTT262640.6746571999568449No Hit
CTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGC160810.413081116071658No Hit
TTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCT143860.36954075839853684No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAA135820.3488879869712865No Hit
GTAATTCTGTCTGAACATGGGGGGACCACCCTCCAAGCCTAAATACTCGT112160.28811129891547266No Hit
CGGCCCCCCATGGTGCCCGGCTTAGCCAGTACCTGACCACGTTCGATTTC111900.2874434232225516No Hit
AGGCTGCCAGGACTCTGCTGGGTTGAAATTTGCCGGGGAGGACTCTTGTC100940.259289894013265No Hit
CGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGAC97240.24978550915246575No Hit
CGGCCCCCCGGTGCTGGAAGGTTAAGGGGAACTGTGAGTGTATGCGTAGC92860.23853437247941142No Hit
GACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACC87780.22548510894080054No Hit
TAAAGACAGCCCTTCTCAAGATTCCTCCGCGCGCAGCGGATAGGGACCGA81420.20914784199088607No Hit
CGGCCCCCCGCTAGACTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTC76720.19707470446500588No Hit
CGGCCCCCCGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA76580.19671507909189456No Hit
CGGCCCCCCGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC69430.1783484975365662No Hit
AGGCGACCGACACGCTGGAGATCGGCCTGTCCGCGCGTAACCTGCTCGAC67330.17295411693989632No Hit
GTTGGTGCTTGTGAAACGATGTTTTGCGCGATAATAGCAGCAGTATTACC64800.16645517269724167No Hit
CGGCCATAGTCCCTCTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAG62310.16005897856119025No Hit
CGGCCCCCCAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAA60440.155255411077489No Hit
TGCGCCACTAAGAGCTCAAGGCTCCCAACGGCTAGTTGACATCGTTTACG59140.15191603261288383No Hit
CGGCCCCCCTAGCACGGGGCCCGTGAAATCCCGTGTGAATCTGTCAGGAC57850.1486023416749295No Hit
TATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCC57460.14760052813554794No Hit
CGGCCCCCCAGCCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGA57320.14724090276243662No Hit
ATACCTCTCTCACACTCAAGACACCCAGTATCAAAGGCAATTCCGAGGTT54770.14069058346648033No Hit
TGGCAATCAGGTGTTGAGTGTAAGTGCACAAGGGAGCTTGACTGTGAGAG54130.13904658176082857No Hit
CGGCCCCCCGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACG52690.13534757792311208No Hit
GTCAGGGTGGTATTATGGGCCCCCAGAGACAGGAAGGCCAAGGCGGTGGA52340.1344485144903338No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT51880.13326688826439656No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCAGCATAG51290.13175132419199886No Hit
CTTGCATAATGGGTCTGTGACTTAGTGTATCAAGCAAGCTTAAGCCGTTA50520.12977338463988655No Hit
GGCTAGTTCATTCGGCCGGTGAGTTGTTACACACTCCTTAGCGGATTCCG50280.12915688400026715No Hit
CGGCCCCCCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCC50240.1290541338936639No Hit
AGCTACCTGTGGAGCAACCTGCTCAGATACATCAAACATGGAGACAGCAC48630.12491844210288368No Hit
CGGCCCCCCAACACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGG46180.11862499807343549No Hit
TGCGTCTTGTTGCAGATTTACCATCAAGTGTAAGTTAAGAGGTTTTTATG45680.11734062174089506No Hit
TGAATCAAGATTTGGGCATGTTCTTGGTCACCATTTCCTGCTACACCAGA45310.11639018325481512No Hit
GAATGAGAGTTTCTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCG45150.11597918282840218No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG44700.11482324412911578No Hit
TGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTT44530.11438655617605202No Hit
AGATTCTCAAAGGCCTCAAGTTCAACCTCACGGAGACTTCTGAGGCAGAA43300.11122699039800253No Hit
CGGCGTTGGCGACAGCGTCGGCCTCGGCGTGGAACTGGAAAACCGCTACA43250.11109855276474848No Hit
AGGCTATCCAGCGTACTCCAAAGATTCAGGTTTACTCACGTCATCCAGCA42750.10981417643220805No Hit
GGAACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAG42500.10917198826593782No Hit
AGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTT41230.10590967238128511No Hit
CGGCATGTGTGATTGTGAAGCACGCCAACCCTTGCGGTGTAGCTATCGGC40720.10459960852209384No Hit
AACTAGGCAAAATGGCACCGTAACTTCGGGAGAAGGTGCGCCGGTGAGGG40210.10328954466290259No Hit
TGGCCCATATGTTATCCAATTACCAAGGTCTTTTGTTGTTATAGCCATTA39850.10236479370347347No Hit
CGGCCCCCCTGCAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGG39300.10095197973767897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)