FastQCFastQC Report
Thu 9 May 2024
EGAF00007335544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892940
Total Bases581.9 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTT225770.5799472891953125No Hit
GTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACGTTCGGAAACGGA172330.44267314677338976No Hit
AGAACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGATGCTTT163700.4205048112737417No Hit
CTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGC135660.3484769865448735No Hit
TTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCT121310.31161538580096276No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAA103570.2660457135224278No Hit
CGGCCCCCCGCTAGACTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTC93020.2389453729058244No Hit
CGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGAC87830.22561354657405458No Hit
AGGCGACCGACACGCTGGAGATCGGCCTGTCCGCGCGTAACCTGCTCGAC84680.2175219756790498No Hit
CGGCCCCCCATGGTGCCCGGCTTAGCCAGTACCTGACCACGTTCGATTTC84020.21582659892009637No Hit
CGGCCCCCCGGTGCTGGAAGGTTAAGGGGAACTGTGAGTGTATGCGTAGC80300.20627083900599547No Hit
TAAAGACAGCCCTTCTCAAGATTCCTCCGCGCGCAGCGGATAGGGACCGA80150.20588552610623334No Hit
GACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACC79300.20370208634091458No Hit
AGGCTGCCAGGACTCTGCTGGGTTGAAATTTGCCGGGGAGGACTCTTGTC75840.1948142021197347No Hit
GTAATTCTGTCTGAACATGGGGGGACCACCCTCCAAGCCTAAATACTCGT69450.1783998725898678No Hit
CGGCCCCCCAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAA59650.15322609647207508No Hit
ATACCTCTCTCACACTCAAGACACCCAGTATCAAAGGCAATTCCGAGGTT58780.1509912816534547No Hit
TGCGCCACTAAGAGCTCAAGGCTCCCAACGGCTAGTTGACATCGTTTACG57550.14783171587540522No Hit
GTTGGTGCTTGTGAAACGATGTTTTGCGCGATAATAGCAGCAGTATTACC53770.13812183080139945No Hit
CGGCCATAGTCCCTCTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAG53590.1376594553216849No Hit
TATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCC50390.12943944679342606No Hit
TGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTT49250.12651106875523382No Hit
CGGCATGTGTGATTGTGAAGCACGCCAACCCTTGCGGTGTAGCTATCGGC47980.12324875287058111No Hit
CGGCCCCCCAGCCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGA46650.11983231182602352No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG45100.11585074519514815No Hit
GGCTAGTTCATTCGGCCGGTGAGTTGTTACACACTCCTTAGCGGATTCCG44080.11323061747676563No Hit
GTCAGGGTGGTATTATGGGCCCCCAGAGACAGGAAGGCCAAGGCGGTGGA43270.11114992781805011No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT42780.10989123901216048No Hit
TGGCAATCAGGTGTTGAGTGTAAGTGCACAAGGGAGCTTGACTGTGAGAG42580.1093774884791443No Hit
CGGCCCCCCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCC41820.10742523645368282No Hit
AGCTACCTGTGGAGCAACCTGCTCAGATACATCAAACATGGAGACAGCAC41620.10691148592066664No Hit
CGGCCCCCCGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC39080.10038685415136117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)