FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139938
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA155340.3752230105861489No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC136950.3308020554897199No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG99940.24140458142126764No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG94670.22867492218482502No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA82780.1999546853117124No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA67980.16420535766477662No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA64780.1564757733086824No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC64670.15621006884644167No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC63430.15321485490845516No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC56840.1372967421251236No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC50480.12193419321738636No Hit
CGGCCCCCCGCGGTTCCGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAG47800.11546066631915744No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG43370.1047600229761895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)