FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303263
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG623011.8860441932719254No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA392511.1882493159036989No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA239850.726100222719172No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC220410.6672493228665111No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC178930.5416765180368623No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG177860.5384372966972355No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC169230.5123116143037959No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA160440.48570156236424405No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC158240.4790414811051981No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT112360.34014851375745736No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC107860.32652562027304516No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG102580.3105414252513348No Hit
CGGCCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATT101730.3079682120376125No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG72770.2202973241912618No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG66640.20173991595582916No Hit
CGGCCCCCCTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAG57190.17313183963856346No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC55610.16834869037070313No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA54800.16589656954350893No Hit
CGGCCCCCCGCGGTTCCGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAG49870.15097193290391955No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG48420.14658233389227562No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC46660.14125426888503884No Hit
CGGCCCCCCATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT46650.14122399578840678No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG46530.14086071862882246No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG45790.13862050947805246No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC43130.13056786577393323No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCCAACACGGGAAACCTCACCCGGC42800.12956885358507633No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT41160.12460406573742387No Hit
NNNNNNNN37070.11222236921492475No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT36280.10983079458099462No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT35820.10843823213592135No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT33330.1009002310745466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)