FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780385
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT939683.3796758362600863No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC153640.5525853433966879No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG125070.4498297897593319No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA89040.3202434195264325No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA88360.31779771506464033No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC73870.2656826302832162No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG73430.2641001156314683No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC70800.2546409939630663No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA69290.24921009140820424No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC67680.24341952643249046No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG65680.23622627801545468No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG60550.21777559582575795No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC59960.21565358754273237No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC54130.19468526840707312No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC49680.17868029067916852No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT49400.17767323590078352No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC48370.1739687129660101No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG43820.15760407281725375No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG41750.1501590607056217No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG39100.1406280065530493No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC38540.1386138969962793No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT38060.1368875173761907No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG37210.1338303867989505No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG36600.1316364460317546No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG36530.13138468233715833No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA36450.13109695240047692No Hit
CGGCCCCCCTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAG29050.10448193325744456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)