FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4296344
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT2921986.8010848293339645No Hit
NNNNNNNN414560.9649134240647397No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC319870.7445167332969613No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC297260.6918905934906516No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG280770.6535091231055986No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG223780.5208614580210523No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC178960.416540202553613No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA177330.4127462791620038No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG175560.40862649731958145No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG165020.3840940110940837No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA158070.36791746657157803No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG154700.36007358814843504No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC146550.3411039711903889No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT134720.313568932096685No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT115700.26929873399336735No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG100320.23350085561118944No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT82470.19195390313252383No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG70470.16402317877711842No Hit
CTCATGGTTCACACCCATGACGAACATGGGGGCGTCAGCAGAGGGGGCAG67170.15634222957938193No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT66210.1541077716309495No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA65940.15347933033295286No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC62060.14444839612470511No Hit
CGGCCCCCCACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTT57790.13450971337490666No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA57320.13341576000431996No Hit
TCATGGTTCACACCCATGACGAACATGGGGGCGTCAGCAGAGGGGGCAGA56830.13227525542647423No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT55830.12994769506352377No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG55670.1295752854054517No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG49650.11556337202048997No Hit
TGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTA47430.11039618801473997No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGTCCAGACTCCTACGGGA46960.10930223464415327No Hit
TTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGT46950.10927895904052376No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG45540.1059970989287636No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGGGCGCAAGCCTGATGCAGCCA44490.10355316054766564No Hit
CGGCCCCCCACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT43590.10145835622101024No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGCACAATGGGCGCAAGCCTG43330.10085319052664313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)