FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867548
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT2872607.427444985815303No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG383530.9916619005116419No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC378660.9790699430233316No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC212130.5484870517444128No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC209580.5418937269815397No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC208440.5389461229699024No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA193390.5000325787811812No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG191550.4952750424816964No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT182460.47177177891521965No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA178370.46119660311908217No Hit
NNNNNNNN171700.44395053403344964No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG150180.3883080442699095No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG142320.3679850902949362No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG140760.36395152691059035No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG132230.3418962091743917No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT125780.325218975950654No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT96670.2499516489517389No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC73130.18908621172898177No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT72920.18854323204262752No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG72790.1882071017605987No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC67720.1750980207614747No Hit
TGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTA65270.1687632577540085No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG63920.1652726740560169No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA62850.1625060632731643No Hit
TTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGT62680.16206650828897276No Hit
CGGCCCCCCACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTT54490.14089030052115709No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGCACAATGGGCGCAAGCCTG52290.1352019419022078No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGGAACTGAGACACGGTCCAGAC50510.13059954265596702No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA50250.1299272820919094No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA49620.12829834303284665No Hit
TCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTG46970.12144645651456686No Hit
CGGCCCCCCTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG41840.1081822384621988No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT41730.10789782053125133No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACTGGAACTGAGACACGGTCCAG41280.1067342926319208No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGTCCAGACTCCTACGGGA41230.1066050117542174No Hit
CGGCCCCCCACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGC40700.10523463445056144No Hit
CGGCCCCCCATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG40430.10453651771096312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)