FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502926
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT3267887.257236738955959No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC482701.0719696481798724No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG349310.7757400410310984No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC268920.5972116796944921No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG236850.5259913220870163No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC220990.4907697794722809No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA200250.44471083912993464No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA196820.4370935698254868No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG167910.3728908714022838No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC152620.33893517237458487No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG152520.33871309455229776No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT142670.3168384290570176No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG128780.28599181954133823No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC128170.2846371448253869No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG105670.23466963481078745No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT97290.21605951330312778No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT96400.21408302068477253No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT82190.1825257621377744No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG80790.1794166726257549No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC74780.1660697955062997No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG70030.15552109894766203No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT68420.15194564600883959No Hit
NNNNNNNN64260.14270720860169586No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA60380.13409058909695606No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGCACAATGGGCGCAAGCCTG57700.12813890345966156No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG57020.12662877426810923No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA56510.125496177374445No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGGAACTGAGACACGGTCCAGAC55570.12340864584494615No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA55500.12325319136934516No Hit
TTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGT49900.11081683332126711No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACTGGAACTGAGACACGGTCCAG49470.10986189868543254No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGTCCAGACTCCTACGGGA49450.10981748312097511No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGACACGGTCCAGACTCCTACGG47860.1062864457466101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)