FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5524120
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT4489068.126289798194101No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG636191.1516585447093834No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT464020.839988993722077No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC312420.5655561428788658No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG266160.4818142980239386No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG251560.4553847490641043No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC247960.4488678739781178No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG193700.350644084487665No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA182250.3299168012280689No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA178070.32234998515600677No Hit
NNNNNNNN162800.2947075733329471No Hit
AGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCG161730.29277061323794557No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG153580.27801713214050383No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC149060.26983483342143183No Hit
TCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTG136330.24679043902015163No Hit
TTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGT131420.23790214550009778No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC129400.23424545447962752No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT123970.2244158345582645No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA118160.21389832226671396No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGTCCAGACTCCTACGGGA114920.2080331346893261No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG113150.20482900443871602No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT110470.19997755298581493No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA102630.18578524724299977No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG94160.17045248836013702No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT91580.16578206121517997No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGACACGGTCCAGACTCCTACGG82760.14981571725451295No Hit
TGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTA82460.14927264433068071No Hit
CGGCCCCCCACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTT79380.14369709564600333No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGCACAATGGGCGCAAGCCTG76730.13889995148548545No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC75130.13600356255838034No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA71590.12959530205716024No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACTGGAACTGAGACACGGTCCAG69560.1259205086058956No Hit
CGGCCCCCCACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT69510.12582999645192355No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG67800.12273448078607996No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC65870.11924071164275939No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTCCAGACTCCTACGGGAGGCA65530.11862522899574955No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCCATGCCGCGTGTATGAAGAA60890.11022570110714466No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC59070.10693105870256257No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC58040.10506650833073865No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGGGCTAAACCATGCACCGAA56950.10309334337414827No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG56350.10200719752648385No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGCACAATGGGCGCAAGCCTGA56010.10139171487947403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)