FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5524120
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT4903848.877142422684518No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG710601.2863587322505665No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT455250.8241131619153821No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC211130.3821966213623165No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG190830.3454486868496702No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG174040.3150547055458607No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC166030.30055465847954066No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA159000.28782864963107246No Hit
TCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTG154960.280515267590132No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA145880.2640782604288104No Hit
AGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCG143100.25904578466796524No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC141770.25663816137230905No Hit
TTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGT139840.2531443922289885No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG124800.22591833631420027No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC122860.2224064647400853No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG109760.19869228039941203No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA103330.18705241739860828No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG90090.16308479902681333No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT88810.1607676878851292No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT85140.15412409578358183No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGTCCAGACTCCTACGGGA75970.13752416674511053No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA75320.13634750874347407No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT71030.12858156593267342No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG67840.12280689050925758No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA61150.11069636430779924No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC60170.10892232608994735No Hit
CGGCCCCCCACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT56610.10247786072713845No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGACACGGTCCAGACTCCTACGG55850.1011020759867635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)