FastQCFastQC Report
Fri 10 May 2024
EGAF00007335583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5040709
Total Bases750 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1417902.8128979474911167No Hit
NNNNNNNN239120.47437771154811753No Hit
CGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTG203800.4043082034689961No Hit
CTAACGCGATAAGTAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACT180330.35774729308912695No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTC167350.3319969472548405No Hit
TCAGCAGAGTTCTCTCAAGCGCCTTGGTCTACTCGACCTGACCACCTGTG154410.30632595533683854No Hit
GGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT150780.29912458743402964No Hit
AAACCAGGCCGCTGCAACTGTTTATTAAAAACATAGCACTCTGCAAACAC149070.2957322075128717No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG148020.29364916721040635No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG139570.2768856523953277No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCGGGGGATGACTTG116950.23201101273650193No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC116050.23022554962010303No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA98430.19527014949682675No Hit
TTCAGCAGAGTTCTCTCAAGCGCCTTGGTCTACTCGACCTGACCACCTGT89350.1772568105002689No Hit
GCGCCACTAAAGCCTCAAGGGCCCCAACGGCTAGTAGACATCGTTTACGG84640.16791288685778133No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG84160.16696063986236856No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA80220.15914427910835557No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC78620.15597012245697975No Hit
CGGCCCCCCTTGAAAAGCCGGGGGATGACTTGTGGATAGGGGTGAAAGGC78190.1551170678569225No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG75440.1496614861123703No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC72090.14301559562355215No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG61570.1221455156407561No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC56800.1126825611238419No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT56320.11173031412842915No Hit
AGGCCTTGACCTTTTCAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGAC56070.11123435215165169No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT55440.10998452797017245No Hit
TGCGCCACTAAAGCCTCAAGGGCCCCAACGGCTAGTAGACATCGTTTACG54130.10738568721185848No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAACTGAAGAGTTTGATCATGGC53810.10675085588158333No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGTCAAGTAATTAAGTGCATGTGG53680.10649295565365903No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT50830.10083898911839584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)