FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3135304
Sequences flagged as poor quality0
Sequence length8-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC159700.5093604958243283No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC120180.38331211263724346No Hit
TGGCAGGTCCGCCTCACCGCGTGTACTTGTCCGGCCGGGCCTTTCCTTCT111530.35572308139816744No Hit
GGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCG84280.26880965928662737No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT74090.23630882364198175No Hit
NNNNNNNN65930.21028263925922333No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT64930.20709315587898336No Hit
AGGCCCAAGTTTCAACTACGAGCTTTTTAACTGCAACAACTTTAATATAC62570.19956597510161692No Hit
GGGCAGGATTTATGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGA57390.18304445119197374No Hit
GAATGAGAGTTTCTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCG56500.18020581098356012No Hit
ATGTCCACGTTCACTCAAGTAACAAGGACTTCTTACACATTTAAAGTTTG56410.17991875747933853No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC35760.11405592567738249No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGACCAAGGAGTCTAACA33400.10652874490001607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)