FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338869
Sequences flagged as poor quality0
Sequence length8-150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT2373917.109922551618528No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG605231.81267968285069No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC290050.8687073377242412No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA282870.8472030498950394No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT252160.7552257965197197No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC238730.7150025951901677No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA207640.6218872318740268No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG173440.5194573372001118No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG153540.4598563166149975No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG144670.4332904345753008No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG128890.38602892176961723No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC121340.36341647426119444No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT111800.3348439246942603No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA106920.320228197033187No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC98030.2936024144702892No Hit
TGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTA92200.27614141195716274No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA85040.2546970246511618No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG80810.24202806399412496No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC76820.23007790961550156No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG75190.22519601697461025No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG61630.18458346224425096No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC58500.1752090303632757No Hit
CGGCCCCCCACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT57680.1727531089120298No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG57090.17098604347759674No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC56790.17008753562957996No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT56450.16906922673516092No Hit
CGGCCCCCCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA51870.15535200692210446No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC51720.15490275299809608No Hit
CGGCCCCCCACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTT49540.148373595969174No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCGACCTCAGATCAGACGTGGCG47480.14220384207945866No Hit
CGGCCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT46420.13902911434979928No Hit
CGGCCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT43210.12941508037601956No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC42930.12857647305120387No Hit
CGGCCCCCCGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAA42890.12845667200480163No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT41930.12558144689114786No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG40290.12066960398865603No Hit
CGGCCCCCCGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCA39090.11707557259658884No Hit
CGGCCCCCCAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT38360.11488920349974796No Hit
CGGCCCCCCATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA36920.11057636582926733No Hit
CGGCCCCCCATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG36420.10907885274923934No Hit
CGGCCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGG36090.10809049411642085No Hit
CGGCCCCCCTGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAA35690.10689248365239847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)