FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5833077
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC828571.4204681337139902No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC820831.4071989792008575No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG621811.0660068433864323No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC205510.35231833901729737No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG183820.3151338478816583No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA152520.2614743470727371No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT144190.24719371954116157No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC133510.2288843435462964No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC131790.22593564254337803No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT128070.219558219444043No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT124120.21278649330362No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT121300.20795199514767249No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG108950.1867796362022994No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCTAAACCTAGCCCCAAACCCAC106800.18309375994865146No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCCATGGCACATGCAGCGCAAGTAG84840.14544639132999615No Hit
ATGGGCCATGGAGGAAGTGTCAGCAAACATGAAAAAGCAAATCCGGCCAT84180.14431491303817864No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG65250.11186205839559464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)