FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632755
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC898021.5942820165265488No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC594181.0548656918328598No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC274380.48711509731916264No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG259990.46156809589623554No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC162350.2882248562204463No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG150130.2665303213081343No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT118550.21046539393245403No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT109770.19487799487107108No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT108350.19235702600237362No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCTAAACCTAGCCCCAAACCCAC81210.14417456466684597No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT77870.13824496183483928No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG69670.12368725428320598No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC65320.11596456796008348No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA64260.1140827179595065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)