FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226431
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGGAACCTTTGGGCGCCTCCGTTACATTTTGGGAGGCAACCGC421861.3075128524366397No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC394291.2220623964994137No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC381791.1833198974346577No Hit
CGGCAGACATGACAAGGAGGGGCCCACAACACCGCACATGCAACCCCTGC297980.9235591897052812No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG185460.5748147101239729No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC97200.30126167272754323No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG87290.2705466194690046No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT82290.2550496198431022No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT80510.2495326879762809No Hit
CGGCCCCCCAAGTTTCCAAGGGCGCATGGTGGATGCCTTGGCAACAGGAG75100.2327649343810545No Hit
CGGCCCCCCATAACCATCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT64010.1983925892108029No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAGTTTCCAAGGGCGCATGGTGG62090.19244174135445638No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT57410.1779365497046117No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC51690.1602079821325793No Hit
CGGCCCCCCGGTGCTGGAAGGTTAAGGGGACCCGTTAGCTTCGGCGAAGC46630.14452501851116606No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG44210.13702447069222926No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT42550.13187946681642967No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA41710.12927597087927806No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC33880.10500766946511485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)