FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4894156
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG400500.8183229141040866No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG279270.5706193263966249No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT202790.4143513202276347No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG185560.3791460672687998No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC127010.2595135913117604No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC118530.24218680401687237No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC117990.2410834472787545No Hit
TACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCT95020.1941499208443703No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG93570.19118720367720193No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT86050.17582193947230124No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG71630.14635822805811666No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTT69240.1414748528653357No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG65490.13381265329507275No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG63960.13068647587040544No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT58270.11906036505579308No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA51050.10430807681651343No Hit
GTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGGG50000.10216266093683979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)