FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4275045
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC183740.4297966454154284No Hit
NNNNNNNN176590.41307167526891525No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC119010.27838303456454844No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG116500.27251175133829No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC105220.24612606417008476No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT89370.2090504310480942No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG81480.19059448497033366No Hit
CGGCTGAATTCATGCCCCTGTGTTAGAAAACACTTGTAGTCCCGGTCTCT68290.15974100857417875No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG68100.15929656880804763No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTT63310.1480920083882158No Hit
AGGCTGCCGCAGTGCCAAGGACAAGACTCGACTGGGGAACCAGAACGCAG44170.10332054984216542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)