FastQCFastQC Report
Thu 9 May 2024
EGAF00007335608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4275045
Total Bases638.9 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC189070.44226435043373813No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC123870.2897513359508496No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC121250.2836227454915679No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG92780.21702695527181584No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT87010.20353002132141298No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG79780.18661791864179209No Hit
CGGCTGAATTCATGCCCCTGTGTTAGAAAACACTTGTAGTCCCGGTCTCT64690.15132004458432602No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG45910.1073906824372609No Hit
CGGCGAGCATATGCCAGTGGAGTCAGGCATCAGGCCCTGGCTTGGAGTAG44310.10364803177510412No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTT43360.10142583294444854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)