FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5807003
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC660791.1379191641540396No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC471190.8114168358445828No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG382660.6589629796988222No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT121990.21007394003412774No Hit
AGGCAAGCACCGGAAGCACCCCGGCGGCCGCGGTAATGCTGGTGGTCTGC116960.20141198480524292No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG96270.16578259043434282No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT93400.16084028198366696No Hit
GCAGAGGCAGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGT89790.15462365009971582No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA84700.14585837134921403No Hit
NNNNNNNN81600.14051998939900667No Hit
CGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAGGATCTC78260.13476831336233167No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG78030.13437223986280014No Hit
TGGCTCCTGTGGAGGCCTGCTGGGAACGGGACTTCTAAAAGGAACTATGT74240.12784563741399824No Hit
CGGCCATGCTTTACGGGCTTGTAGGTGATGGAGAACTCGCCCAGGTAGTG69930.12042356444451638No Hit
CGGCCCCCCGCTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAA66190.11398306493039526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)