FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5726423
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC250460.43737600243642494No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC181000.31607864106441314No Hit
CCTTTCTGTTCCTCTCAAGATGCTTTCGAACAGCAACTGCTTTCTTAATT169700.29634555463331996No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC169630.2962233142748973No Hit
GCAGAGGCAGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGT146270.25542996037840726No Hit
AGGCCTTGACCGCGTACTTCCGCAGCGGGTACAGCCGCTCCTTCCGCTGC145530.2541377051607958No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG138360.241616799876642No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG116830.20401915820748834No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA114010.19909461805388812No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG100120.17483863836115493No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC99570.17387817840211944No Hit
AGGCCACAGGTTATCCACTGGCTTATGTGGCAGCCAAGCTAGCATTGGGC99550.17384325258542724No Hit
NNNNNNNN98560.17211442465916332No Hit
AGGCCAACCCTTTTGGAGGTGCTTCTCATGCAAAAGGAATCGTGCTGGAA95180.16621196163818147No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT92660.16181130873496422No Hit
CTCTAACTCCTGAGCTCAAGTGATCTGCCCTCCTCAGTCTCCCAAAGTGT88160.15395299997921913No Hit
AGGCTGAGCAGCTGCTACGCGGTCCTCTGGGTGATCAGTACCAGACGGTG87600.15297507711183753No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT83360.14557080397309105No Hit
GACAGTGAGACTGCCTCAAGAAAAAACAAAAAAGAGTCAGCCCTAGTGAT83080.14508184253940026No Hit
CGGCCCCCCGCTCTCGGCTTTCGGCTCGGAGGAGGCCAAGGTGCAACTTC81860.14295136772117603No Hit
CGGCCATGCTTTACGGGCTTGTAGGTGATGGAGAACTCGCCCAGGTAGTG80750.1410129848947589No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA80110.1398953587606085No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC72420.12646638224245746No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT71350.12459785104942474No Hit
CGGCCCCCCGCCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCA67760.11832866695317477No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCTCTCGGCTTTCGGCTCGGAGG67560.11797940878625279No Hit
GGGCAGGCACATGGGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAA66810.11666969066029527No Hit
AGGCCTTGACCTTTTCAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGAC66180.11556952743449096No Hit
AGGCTGCAAGCTGCACCCTCCCCACTTCCCTGTCCAGCTGAGTCCGTTTG64220.11214679739865532No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTT64070.11188485377346383No Hit
AGGCAGCAGGGTTCCACCTGAATGAGCATCTCTATAACATGATCATCCGA62960.10994647094704671No Hit
CGGCCCCCCGGCTTGTGCAGCAATGGCCAAGATCAAGGCTCGAGATCTTC62750.1095797498717786No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC62620.10935273206327928No Hit
AATAAAGCCCAAATCTCAAGCGGTGCTTGAAGGGGAGGGAAAGGGGGAAA62450.10905586262139558No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG61720.10778107031213027No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT58690.10248980908326193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)