FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5726423
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC221600.3869780489495799No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC153650.2683175867378292No Hit
GCAGAGGCAGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGT145920.25481875858629377No Hit
CCTTTCTGTTCCTCTCAAGATGCTTTCGAACAGCAACTGCTTTCTTAATT138750.24229785330213993No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG135270.23622076119769708No Hit
AGGCCTTGACCGCGTACTTCCGCAGCGGGTACAGCCGCTCCTTCCGCTGC125690.2194912950021331No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC110130.19231900961560122No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC101930.1779994247717991No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA93730.16367983992799695No Hit
CTCTAACTCCTGAGCTCAAGTGATCTGCCCTCCTCAGTCTCCCAAAGTGT87100.1521019316945325No Hit
CGGCCATGCTTTACGGGCTTGTAGGTGATGGAGAACTCGCCCAGGTAGTG86780.1515431186274573No Hit
AGGCTGAGCAGCTGCTACGCGGTCCTCTGGGTGATCAGTACCAGACGGTG82020.14323077425471362No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA80340.1402970056525688No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG79870.1394762489603021No Hit
CGGCCCCCCGCTCTCGGCTTTCGGCTCGGAGGAGGCCAAGGTGCAACTTC78350.13682188689169486No Hit
GACAGTGAGACTGCCTCAAGAAAAAACAAAAAAGAGTCAGCCCTAGTGAT77070.13458663462339404No Hit
CGGCCCCCCGCCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCA74890.13077972060394422No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT72430.12648384515080358No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT70330.12281663439812254No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC69470.12131482428035792No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG69360.1211227322885508No Hit
AGGCCACAGGTTATCCACTGGCTTATGTGGCAGCCAAGCTAGCATTGGGC68560.1197256996208628No Hit
CGGCCCCCCGGCTTGTGCAGCAATGGCCAAGATCAAGGCTCGAGATCTTC65980.11522026926756895No Hit
AGGCCTTGACCTTTTCAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGAC64370.11240874102384682No Hit
CGGCCCCCCACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATT64310.11230396357377022No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT63380.1106799130975829No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG57830.10098799896549732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)