FastQCFastQC Report
Fri 10 May 2024
EGAF00007335623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5766283
Total Bases859.9 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG2345224.067126084515796No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC1207112.093393612488322No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT1020021.7689384998967272No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG642381.1140278754962252No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG622801.080071859116176No Hit
TACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCT477190.8275521683552473No Hit
GTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGGG416500.7223023913325101No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA364340.6318455060218168No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG352580.6114510855606636No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC311970.5410244346314602No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT288510.5003396468747718No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC282320.48960482862183485No Hit
GCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACGG280120.48578954588250356No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC246890.4281614343243299No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT232920.40393438892957556No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG220110.3817190380701051No Hit
GCAGAGGCAGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGT166650.2890076675043525No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA158880.2755327825568048No Hit
NNNNNNNN136040.23592321084483717No Hit
CGGCCCCCCGTCAAGTAATGAAGTGCACATGGTGGATGCCTTGGCAGTCA125890.2183208836611037No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT125530.2176965646673949No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAAACTGAAGAGTTTGATCATGG124680.21622247815447143No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT114170.19799583197702922No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG109870.19053868844106334No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC106150.18408739217273934No Hit
GGGCAGGCACATGGGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAA100800.17480931823845622No Hit
AGGCAAGCACCGGAAGCACCCCGGCGGCCGCGGTAATGCTGGTGGTCTGC100620.17449715874160182No Hit
CGGCCCCCCTAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGC99940.17331788953126304No Hit
CGGCCCCCCGAGGCTAATGGTTAATACCCATTAGATTAGACGTTACCTGC95150.16501097847608243No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGTCAAGTAATGAAGTGCACATGG84700.14688838546425836No Hit
CGGCGAGGCACTGTAGTATCAGTCAGTCCAGGAATATCCTTCTCTCCTTT83460.144737953374817No Hit
CCTTTCTGTTCCTCTCAAGATGCTTTCGAACAGCAACTGCTTTCTTAATT82900.1437667904957145No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG82900.1437667904957145No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGCGAGCGTTAATCGGAATTACT82290.14270891664526353No Hit
CGGCCCCCCGCAACCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCT81290.1409746972182947No Hit
CGGCCATGCTTTACGGGCTTGTAGGTGATGGAGAACTCGCCCAGGTAGTG79890.13854679002053835No Hit
CGGCCCCCCGCGCAAGCTCTTGCTATTAGATGAGCCTAAATCAGATTAGC78550.13622293598840016No Hit
CGGCCCCCCTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA74320.12888718781232208No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTT70830.12283476201220093No Hit
AGGCGTTCAGCTGCTTCAAGATGAAGCTGAACATCTCCTTCCCAGCCACT70630.12248791812680716No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC68710.11915821682702704No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC67880.11771881470264292No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGAGGCTAATGGTTAATACCCATT67560.1171638644860129No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT67430.11693841596050697No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCTCTCGGCTTTCGGCTCGGAGG66500.11532559189342598No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG65860.11421569146016593No Hit
CGGCCCCCCGCTCTCGGCTTTCGGCTCGGAGGAGGCCAAGGTGCAACTTC63570.11024432897240735No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCGCAAGCTCTTGCTATTAGATG61060.10589143821071564No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC60980.10575270065655813No Hit
CGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCC58230.10098359723239389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)