FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5766283
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG2331374.043107145452279No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT896151.5541207394781007No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC676391.1730086782074345No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG450770.7817340910947311No Hit
TACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCT398800.6916067074751621No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG354510.6147981290547134No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT313830.5442500827656221No Hit
GCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACGG284770.49385366621790844No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC278750.48341366526755625No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA273150.47370203647653086No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT268080.464909543981799No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC244690.42434615158499855No Hit
GTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGGG238830.41418362574296125No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG232800.40372628259833937No Hit
GCAGAGGCAGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGT169650.2942103257852589No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG152190.2639308545903834No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA136390.23653018764427625No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC129080.2238530436331342No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT101600.17619669378003128No Hit
CGGCCCCCCGTCAAGTAATGAAGTGCACATGGTGGATGCCTTGGCAGTCA95060.16485489872765524No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT93540.16221888519866262No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC89950.1559930374558446No Hit
CGGCCCCCCTAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGC81900.14203257106874567No Hit
CGGCCATGCTTTACGGGCTTGTAGGTGATGGAGAACTCGCCCAGGTAGTG81390.1411481191609916No Hit
CGGCCCCCCGAGGCTAATGGTTAATACCCATTAGATTAGACGTTACCTGC79700.13821728832941427No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC75300.1305867228507515No Hit
CGGCGAGGCACTGTAGTATCAGTCAGTCCAGGAATATCCTTCTCTCCTTT74950.12997974605131243No Hit
CGGCCCCCCGCAACCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGCCT71230.12352844978298844No Hit
CCTTTCTGTTCCTCTCAAGATGCTTTCGAACAGCAACTGCTTTCTTAATT70900.12295615737208876No Hit
CGGCCCCCCTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA70380.12205436327006496No Hit
GGGCAGGCACATGGGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAA69680.12084040967118678No Hit
AGGCGTTCAGCTGCTTCAAGATGAAGCTGAACATCTCCTTCCCAGCCACT69030.11971316704365706No Hit
CGGCCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGT65620.11379947879769342No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG64770.11232539228476994No Hit
CGGCCCCCCGCTCTCGGCTTTCGGCTCGGAGGAGGCCAAGGTGCAACTTC62980.10922113951049575No Hit
CGGCCCCCCGCGCAAGCTCTTGCTATTAGATGAGCCTAAATCAGATTAGC60770.10538851457689469No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGCGAGCGTTAATCGGAATTACT59130.10254439471666582No Hit
AGGCAAGCACCGGAAGCACCCCGGCGGCCGCGGTAATGCTGGTGGTCTGC58020.10061941115273045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)