FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5323473
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC352570.6622932059578399No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC203530.38232559834529073No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC142090.26691222064994036No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG111660.20975028895610068No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG89880.1688371482301122No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG83650.15713426178737075No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT81860.1537717952171449No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA69600.13074171692051412No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG68960.12953949423618755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)