FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593214
Sequences flagged as poor quality0
Sequence length8-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC368720.659227413791069No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC188180.33644341160556346No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC181990.3253764293660139No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG122480.21897964211632168No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT112700.20149416775399617No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG81230.1452295585328936No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA70490.12602771858899015No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG67750.12112892515823638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)