FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5777401
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC385480.6672204335478877No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG255310.4419115100371257No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC244980.42403149790018035No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG207710.3595215218746284No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC181020.3133242785120853No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG157920.27334090190381455No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT130030.2250666000161664No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTT108000.18693526725944765No Hit
NNNNNNNN107870.18671025258589458No Hit
TGGCCGCCTTAGCGGTAGTAACTTTGTGTTATGAATCACATGAAAGCATG82930.14354205290579622No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA76770.13287981914359068No Hit
TGCTTCACCACCTTCTTGATGTCATCATATTTGGCAGGTTTTTCTAGACG68130.11792499776283488No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC65860.11399589538617796No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG64360.11139957222979675No Hit
GTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACT58100.10056425025716581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)