FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5777401
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC356780.6175441171557937No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC216720.37511676963395824No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC203170.3516633171213146No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG179720.3110741317765549No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG155010.268304034980435No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG146680.25388578705199794No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT114740.19860141264212056No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTT76660.13268942211212273No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA70110.12135214432925807No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG69850.120902114982152No Hit
TGGCCGCCTTAGCGGTAGTAACTTTGTGTTATGAATCACATGAAAGCATG64070.11089761641956307No Hit
TGCTTCACCACCTTCTTGATGTCATCATATTTGGCAGGTTTTTCTAGACG62790.10868208732611774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)