FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6438455
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTG265400.41221069340392996No Hit
GGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT220090.3418366673371174No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC217930.3384818252204916No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC164720.2558377747456494No Hit
GCGCCACTAAAGCCTCAAGGGCCCCAACGGCTAGTAGACATCGTTTACGG119020.18485801329666823No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT108290.16819252444880023No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG108060.16783529589008542No Hit
TCAGCAGAGTTCTCTCAAGCGCCTTGGTCTACTCGACCTGACCACCTGTG102200.15873373348109135No Hit
AAACCAGGCCGCTGCAACTGTTTATTAAAAACATAGCACTCTGCAAACAC84440.13114947607772362No Hit
TGCGCCACTAAAGCCTCAAGGGCCCCAACGGCTAGTAGACATCGTTTACG81300.1262725296674435No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA81100.1259618961381263No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC79020.1227313074332274No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG78280.12158196337475373No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTT71680.111331056907286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)