FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6099351
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNN1317212.1595904219973567No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC187710.3077540544887481No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG128020.20989118350460564No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC113180.18556072605101756No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC104060.1706083155404567No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG83120.13627679403923468No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC75900.1244394690517073No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT75620.12398040381673395No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTT73350.12025869637605706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)