FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7064057
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNN765071.0830461871980932No Hit
CGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTG208880.29569410326105805No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC194950.2759745568304446No Hit
GGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT175020.24776130770179233No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC140280.19858276908014758No Hit
TGGCTGTACAGGGCAGATCTGATTGGATCTCCTAAGAGCAGGAGTGAGCT130490.18472387751118088No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC124240.17587627053405713No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG121640.17219566603157366No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT104640.148130175053797No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTC96310.13633808447468643No Hit
CTAACGCGATAAGTAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACT92880.1314825177656409No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG92860.13145420542331412No Hit
TGGCCTCCGCCTGTGGTTTACCTGTAATAGCAAACACAGGGCATGGCTGT75800.10730377741855707No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTT74600.10560503687894932No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG73670.1042885129607533No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG73320.10379304697003436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)