FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5672224
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNN1251212.2058543527194976No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC375370.6617686466542929No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC361010.6364522980756755No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG275080.4849596912956893No Hit
GGGCCGACTCACCCTGCGCCGATTAACGTTGCGCAAGGAAACCTTGGGCT195460.34459146888416253No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG130450.2299803392813824No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC110490.1947913199478723No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA108570.19140640426048053No Hit
TGGCTGCTGCTGGAGCTGCTCAGGTAGCCCTCCTGGTCCGAGAGAGAATC95980.16921052483117732No Hit
TGGCTCCTGTGCGCTTCAGGCACTCCCCGGTCCTGTCCTCAAAAGGGATT93200.16430944899214134No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC88500.15602345746571364No Hit
TGGCCTCCTTCTTTCCGTAATCTGTTGGCAGCAGCCATGACCAACCTGCA79160.13955725302808916No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT76730.13527321911123397No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG75210.13259349419204883No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT73290.12920857850465708No Hit
AGGCATGTGGGGTGCCCAGCAAAGGGTAACAGTGGACAGTTGTTGCCTCA72300.12746323135334572No Hit
AGGGGAGCTAGATGTGAATGACAGAGCAAGCGGCTAGCTGAAGAAAGCTT71310.12571788420203434No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG70330.12399016681992814No Hit
TGGCAGCTGTGGTGCGGGGTTCCAGCCAGCGGGCGTTGACAGTGGCCAGC67000.11811945367460805No Hit
TGGCAGAGGACATATGTGGAGGTGGTTCATCAGAGTGTCGCACAGAACTC65730.11588047298555204No Hit
AGGCAAGCACCGGAAGCACCCCGGCGGCCGCGGTAATGCTGGTGGTCTGC63120.11127910322300388No Hit
GCAGAGGCAGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGT62600.11036235522433528No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC61310.10808811499686895No Hit
TGGCCCCGACGCCTCTGTTCTCGGAATCCGGGTGCTGCGGATTGAGGTCC61300.1080704852276638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)