FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5182849
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC550431.062022065470169No Hit
NNNNNNNN451840.8717985031012865No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC266570.5143310175542448No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT215590.4159681287261119No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG212250.4095237966608713No Hit
GGGCCAGGTCAGCCTTGGCTTGCTCTGTCTTCCCGGCCAAGTGCATTTTC129640.2501326972867625No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC95350.18397217437745148No Hit
AGGCAAGCACCGGAAGCACCCCGGCGGCCGCGGTAATGCTGGTGGTCTGC94960.18321969248959402No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG84490.16301844796172915No Hit
GCAGAGGCAGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGT76580.14775657172339No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG71360.13768489107052898No Hit
TGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTTAAACCCTTCCTGGGC70120.13529238455528997No Hit
AGGCTCTCATCGCTGCTCAGTACAGCGGGGCTCAGGTCCGCGTGCTCTCC69290.1336909487426703No Hit
CGGCCCCCCACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATT68250.13168433037505048No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA67960.13112479256100265No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC67580.13039160508052616No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC66570.12844286993504925No Hit
TTGATGCTTGACTGCTCAAGCTCTTCATCTTCAAATGCTCCATCACAAAG65810.1269764949740963No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGCAGGCTGAGAAGCAGAAGGCC64190.1238508009783808No Hit
TGGCCTGCACCCTTTCCCCTTTCATCCGAGCCTGGGGCCCCTGGAGCGAG61870.11937449846599814No Hit
CAATTGCTTTATCAAGATCATCTTGGTTTTTCAGCAGGATGGAGAGCTCA61210.11810106757885479No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA60850.11740646891314024No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG60530.11678904787694953No Hit
TGGCTTGGCCAGTCTTGGCATTCTCATCAAACTTGTCAGCAGTGACAAAG58210.11231274536456685No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA57990.11188826840218574No Hit
GGGCCTCCACACCAGAGGCCCTGATGAACTCCCCGGCCAGGGCGTCGTAT57570.11107790329218543No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACTCCATGGAGCAGCTGCTGCG55150.10640865670599317No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT54230.10463357122694487No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT52410.1011219890836102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)