FastQCFastQC Report
Wed 8 May 2024
EGAF00007335659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5134938
Total Bases756.8 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNN757821.4758113924647192No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC355150.6916344462192143No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC246750.48053160525015104No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG186490.3631786790804485No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC97250.18938884948562185No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG82520.16070301140929064No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC81460.15863872163597692No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG68460.13332196026514828No Hit
GTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACT66370.129251804013992No Hit
CGGCCCCCCACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATT66210.12894021310481255No Hit
GCCATAGCTAGGCCACCAATCAGATTAAGAAATTCTGAGAAATCTAGCTG64520.12564903412660486No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT57990.11293223014571939No Hit
ATCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTT56810.11063424719052109No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG52080.10142284093790423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)