FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869661
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATCTGCCCAGGTCTGGGACCCCGCACCGTGGGGTTCGCATGGCCTT419461.0839709214838198No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC350130.9048079405405279No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC290920.7517971212465381No Hit
CCTTTGAGGGCGACCTCAAGTGGCACCACCACAACATCACCTATTGGATC240100.6204677877467819No Hit
CGGCTCCCAGGCCCAGCTTGTCCAGACGCCTCGGGCCCAGCACCGACGCG148870.3847107020485774No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG143330.3703942024895721No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC122150.3156607258361908No Hit
TTGGAATCTGCCCAGGTCTGGGACCCCGCACCGTGGGGTTCGCATGGCCT98880.2555262592769754No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGACACCTCTGCCCTCACCATGA91020.23521440250192457No Hit
CTTTGAGGGCGACCTCAAGTGGCACCACCACAACATCACCTATTGGATCC83560.21593622800550227No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG74060.19138627388807442No Hit
CGGCCCCCCAGACACCTCTGCCCTCACCATGAGCCTCTGGCAGCCCCTGG72140.18642459895065744No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT71300.1842538661655375No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT70330.1817471866398633No Hit
TGGCCCAGGTGACCGGGGCCCTCCGGAGTGGCAGGGGGAAGATGCTGCTG67860.17536419856933202No Hit
ACAGCTGTCCTGGCTCAAGAAACAGCTGGCGGCGGCCAGGGAGGACTACG64190.1658801636629152No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT61380.15861854565554967No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT59390.15347597631937268No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG50160.12962375774001908No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGAGACACCTCTGCCCTCACCATG48410.12510139777101922No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA48210.12458455663170495No Hit
TGGCCAGCTTCCCCAAGATGTGACTCCAGCCAGCCAAATCCATCCTGCTC47700.12326661172645356No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC46330.11972624992215081No Hit
TGGCCCTACCAACTTTGCACCCATCATCAACCATGTGGCCAGGTTTGCAG42950.11099163466773963No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG42310.10933774302193397No Hit
CGGCCCCCCGAGACACCTCTGCCCTCACCATGAGCCTCTGGCAGCCCCTG39680.10254128203995129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)