FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007356541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007356541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30555591
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA974101231.879638655982795TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGACCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1056110.3456355990627051TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA813170.26612805492781993TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT759960.24871389331006555TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG707010.23138482250269682TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAACAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA538060.17609215936945877TruSeq Adapter, Index 12 (97% over 35bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA315010.10309406222906964TruSeq Adapter, Index 12 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)