FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9693846
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTATGTTCATCTCGTATG106871111.024633566491566TruSeq Adapter, Index 21 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTATGTTCATCTCGTAT3336023.4413792007836723TruSeq Adapter, Index 21 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTATGTTCAACTCGTATG161420.16651801565653096TruSeq Adapter, Index 21 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTATCATTGATCGGAAGA129440.13352801354591357TruSeq Adapter, Index 21 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)