FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9693846
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCCTGTTGTGTAGATCTC6765236.978891556560729Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCCTGTTGTGTAGATCT2563152.6441001847976544Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGACGTGTAGGGAAAGAGTGTTTCCTGTTGTGTAGATCTC183180.1889652466110974Illumina Single End PCR Primer 1 (96% over 31bp)
ATGATACGGCATACGAGATGAACATACGTGACTGGAGTTCAGACGTGTGC143290.14781542846874193TruSeq Adapter, Index 19 (96% over 26bp)
ATCGGAAGGGCGTCGTGTAGGGAAAGAGTGTTTCCTGTTGTGTAGATCTC117250.12095302524921482Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGAGTAGGGAAAGAGTGTTTCCTGTTGTGTAGATCTC116240.11991112712126847Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)