FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649190
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCACCTATCTCGTATG350284523.91152684892475TruSeq Adapter, Index 15 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCACCTATCTCGTAT6679094.559357889412316TruSeq Adapter, Index 15 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCACCTAACTCGTATG438130.299081382656652TruSeq Adapter, Index 15 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCACCTAGCTCGTATG248080.16934724718568056TruSeq Adapter, Index 15 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCACCTACCTCGTATG238100.16253458382340594TruSeq Adapter, Index 15 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)