FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007358375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007358375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649190
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCACCGTGTAGATCTC271376518.525017424171576Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCACCGTGTAGATCT5784183.948464044769711Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGGGCGTCGTGTAGGGAAAGAGTGTCCTTCACCGTGTAGATCTC513100.35025827366564294Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGAGTAGGGAAAGAGTGTCCTTCACCGTGTAGATCTC189000.1290173722915738Illumina Single End PCR Primer 1 (96% over 31bp)
ATGATACGGCATACGAGATAGGTGCGTGTGACTGGAGTTCAGACGTGTGC188260.12851222490799832TruSeq Adapter, Index 25 (96% over 26bp)
ATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTCCTTCACCGTGTAGATCTC173890.11870280882424215Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)